2DU0

Crystal structure of basic winged bean lectin in complex with Alpha-D-galactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	298
Detector technology	IMAGE PLATE
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	157.552, 89.957, 73.404
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.700
R-factor	0.194
Rwork	0.194
R-free	0.24500
Starting model (for MR)	1wbl
RMSD bond length	0.007
RMSD bond angle	1.400
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.114	0.316
Number of reflections	29164
<I/σ(I)>	16.8	4.2
Completeness [%]	99.2	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	300	20% PEG 4000, 10% Iso-propenol, 0.02M PBS, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 300K