2DTN
Crystal structure of Helicobacter pylori undecaprenyl pyrophosphate synthase complexed with pyrophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-03-05 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.431, 58.763, 154.185 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| Rwork | 0.214 |
| R-free | 0.29330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2d2r |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.157 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.059 | 0.159 |
| Number of reflections | 15618 | |
| <I/σ(I)> | 42.3 | 5.2 |
| Completeness [%] | 96.0 | 91.2 |
| Redundancy | 8.83 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 298 | 0.15M KSCN, 15% PEG600, 2% PEG5KMME, pH 8.0, VAPOR DIFFUSION, temperature 298.0K |
