2DT8

Fatty Acid Binding of a DegV family Protein from Thermus thermophilus

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-17
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9791, 0.9000, 0.9794
Spacegroup name	C 1 2 1
Unit cell lengths	105.548, 36.969, 81.461
Unit cell angles	90.00, 127.06, 90.00

Refinement procedure

Resolution	42.110 - 1.480
R-factor	0.173
Rwork	0.173
R-free	0.19700
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.367
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.110	1.530
High resolution limit [Å]	1.480	1.480
Rmerge	0.045	0.194
Total number of observations		15368
Number of reflections	41410
<I/σ(I)>	12.6	3.5
Completeness [%]	97.5	94.3
Redundancy	2.779	2.46

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	10% PEG 3350, 0.05M Zn Acetate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	8	293	10% PEG 3350, 0.05M Zn Acetate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	8	293	10% PEG 3350, 0.05M Zn Acetate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K