2DT4

Crystal structure of Pyrococcus horikoshii a plant- and prokaryote-conserved (PPC) protein at 1.60 resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-17
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9794, 0.9796, 0.9744, 0.9843
Spacegroup name	P 63 2 2
Unit cell lengths	53.922, 53.922, 159.181
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.600
R-factor	0.16531
Rwork	0.164
R-free	0.19320
Structure solution method	MAD
RMSD bond length	0.022
RMSD bond angle	1.519
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall
Low resolution limit [Å]	18.830
High resolution limit [Å]	1.600
Number of reflections	17768
Completeness [%]	95.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	277	1.8M ammonium sulphate, 0.2M potassium sodium tartrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K