2DT3

Crystal structure of the complex formed between goat signalling protein and the hexasaccharide at 2.28 A resolution

Replaces:  2B2ZReplaces:  1ZU6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2005-05-10
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	62.300, 66.420, 106.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.000 - 2.280
R-factor	0.196
Rwork	0.196
R-free	0.21500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ljy
RMSD bond length	0.007
RMSD bond angle	1.500
Data reduction software	AUTOMAR
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.000	2.320
High resolution limit [Å]	2.280	2.280
Number of reflections	21698
<I/σ(I)>	8	2
Completeness [%]	99.5	96.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	298	Precipitant 19% Ethanol, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K