2DT2

Crystal structure of the complex formed between goat signalling protein with pentasaccharide at 2.9A resolution

Replaces:  2B40Replaces:  2A0O

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2005-05-14
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	62.540, 66.470, 107.333
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.000 - 2.900
R-factor	0.195
Rwork	0.195
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ljy
RMSD bond length	0.010
RMSD bond angle	1.900
Data reduction software	AUTOMAR
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.000	2.940
High resolution limit [Å]	2.900	2.900
Number of reflections	10542
<I/σ(I)>	8	2
Completeness [%]	99.1	99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	298	25MM TRIS HCL, 50MM NACL, 19% Ethanol, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K