2DSO

Crystal structure of D138N mutant of Drp35, a 35kDa drug responsive protein from Staphylococcus aureus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-15
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9000
Spacegroup name	P 1 21 1
Unit cell lengths	76.548, 182.432, 81.454
Unit cell angles	90.00, 115.58, 90.00

Refinement procedure

Resolution	19.990 - 2.100
R-factor	0.168
Rwork	0.168
R-free	0.20200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dg1
RMSD bond length	0.005
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.100
Number of reflections	116543
Completeness [%]	100.0
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	293	100mM HEPES, 1M succinic acid, 1% polyethylene glycol mono methyl ether 2000, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K