2DRS
Crystal structure of reducing-end-xylose releasing exo-oligoxylanase D263S mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-22 |
| Detector | ADSC QUAMTUM 4r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.249, 85.026, 86.822 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.400 - 2.100 |
| R-factor | 0.1437 |
| Rwork | 0.140 |
| R-free | 0.21041 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1wu4 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.406 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.746 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 22673 | |
| <I/σ(I)> | 15.2 | 5.9 |
| Completeness [%] | 97.3 | 99.2 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | PEG4000, SODIUM ACETATE, GLYCEROL, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
