2DRP
THE CRYSTAL STRUCTURE OF A TWO ZINC-FINGER PEPTIDE REVEALS AN EXTENSION TO THE RULES FOR ZINC-FINGER/DNA RECOGNITION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS-NICOLET |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.700, 64.600, 117.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.800 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.760 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.800 * |
| Rmerge | 0.056 * |
| Number of reflections | 11994 * |
| Completeness [%] | 99.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6 | 20 * | pH 6.00, SMALL TUBES |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MES | ||
| 4 | 1 | 1 | NACL | ||
| 5 | 1 | 1 | SPERMINE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MES | 20 (mM) | |
| 2 | 1 | reservoir | 5-20 (mM) | ||
| 3 | 1 | reservoir | spermine | 1.75-3.5 (mM) |
