2DRJ
Xray structure of alpha-2,3/8-sialyltransferase CstII F91Y mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-11-23 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.514 |
| Spacegroup name | I 4 |
| Unit cell lengths | 116.653, 116.653, 44.086 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.810 - 2.250 |
| R-factor | 0.197 |
| Rwork | 0.194 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ro7 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.788 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.066 | 0.185 |
| Number of reflections | 13458 | |
| <I/σ(I)> | 23.1 | |
| Completeness [%] | 94.3 | 65.4 |
| Redundancy | 3.9 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 283 | 0.1M TEA pH 7.5, 20% PEG 400, 10% isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
