2DRE
Crystal structure of Water-soluble chlorophyll protein from lepidium virginicum at 2.00 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2003-11-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.060, 82.730, 121.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.990 - 2.000 |
| R-factor | 0.243 |
| Rwork | 0.243 |
| R-free | 0.28000 |
| Structure solution method | MIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.990 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.068 | 0.533 |
| Number of reflections | 50390 | |
| <I/σ(I)> | 9.1 | 1.6 |
| Completeness [%] | 99.4 | 99 |
| Redundancy | 7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1M SODIUM/POTASSIUM PHOSPHATE, 3.2M AMMONIUM SULFATE, 5% SUCROSE, pH 6.00, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
