2DRB
Complex structure of CCA-adding enzyme with tRNAminiCCA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 58.010, 58.010, 428.513 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.800 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 18344 | |
| Completeness [%] | 100.0 | 94.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 50mM HEPES, 20% PEG 3550, 0.2M Tri-lithium citrate, 80mM (NH4)2SO4, pH 7.5, VAPOR DIFFUSION, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | HEPES | ||
| 10 | 1 | 2 | water | ||
| 2 | 1 | 1 | PEG 3550 | ||
| 3 | 1 | 1 | Tri-lithium citrate | ||
| 4 | 1 | 1 | ammonium sulphate | ||
| 5 | 1 | 1 | water | ||
| 6 | 1 | 2 | HEPES | ||
| 7 | 1 | 2 | PEG 3550 | ||
| 8 | 1 | 2 | Tri-lithium citrate | ||
| 9 | 1 | 2 | ammonium sulphate |
