2DR1
Crystal structure of the PH1308 protein from Pyrococcus horikoshii OT3
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-03-17 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 0.978959, 0.979320, 1.0 |
Spacegroup name | P 65 |
Unit cell lengths | 181.238, 181.238, 47.289 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 1.900 |
R-factor | 0.18 |
Rwork | 0.180 |
R-free | 0.20200 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.088 | 0.311 |
Number of reflections | 70704 | |
<I/σ(I)> | 12.7 | 6.62 |
Completeness [%] | 99.9 | 99 |
Redundancy | 8.1 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7.5 | 295 | magnesium chloride, PEGMME550, HEPES, pH 7.5, microbatch, temperature 295K |
1 | MICROBATCH | 7.5 | 295 | magnesium chloride, PEGMME550, HEPES, pH 7.5, microbatch, temperature 295K |
1 | MICROBATCH | 7.5 | 295 | magnesium chloride, PEGMME550, HEPES, pH 7.5, microbatch, temperature 295K |