2DQF
Crystal structure of hyhel-10 FV mutant (y33ay53a) complexed with hen egg lysozyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 98.922, 98.922, 147.211 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 8.000 - 2.500 |
R-factor | 0.224 |
Rwork | 0.224 |
R-free | 0.26800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c08 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.000 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.055 | 0.252 |
Number of reflections | 29457 | |
<I/σ(I)> | 9.6 | 2.9 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 5.8 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | AMMONIUM SULFATE, PEG4000, SODIUM ACETATE, pH 4.60, VAPOR DIFFUSION, SITTING DROP, temperature 293K |