2DQC

Crystal structure of hyhel-10 FV mutant(Hy33f) complexed with hen egg lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Spacegroup name	P 41 21 2
Unit cell lengths	56.480, 56.480, 234.998
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.19
Rwork	0.190
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1c08
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.800	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.062	0.192
Number of reflections	36200
<I/σ(I)>	8	3.8
Completeness [%]	99.2	99.2
Redundancy	7.8	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	HEPES, MPD, PEG6000, pH 7.50, VAPOR DIFFUSION, SITTING DROP, temperature 293K