2DQB
Crystal structure of dNTP triphosphohydrolase from Thermus thermophilus HB8, which is homologous to dGTP triphosphohydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-02-17 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 |
| Unit cell lengths | 74.100, 84.200, 108.000 |
| Unit cell angles | 110.20, 109.70, 94.40 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| Rwork | 0.223 |
| R-free | 0.28600 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.083 | 0.319 |
| Number of reflections | 126444 | |
| <I/σ(I)> | 11.6 | 3.2 |
| Completeness [%] | 84.9 | 72.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.08M Tris-HCl, 0.35M magnesium chloride, 16%(w/v)polyethylene glycol 4000, 19%(w/v) glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
