2DQA

Crystal Structure of Tapes japonica Lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-04
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.97904
Spacegroup name	P 1 21 1
Unit cell lengths	42.749, 88.760, 43.644
Unit cell angles	90.00, 116.04, 90.00

Refinement procedure

Resolution	33.860 - 1.600
R-factor	0.1764
Rwork	0.175
R-free	0.20994
Structure solution method	MIR
RMSD bond length	0.006
RMSD bond angle	1.082
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.062	0.308
Number of reflections	36411
<I/σ(I)>	9.4	2.3
Completeness [%]	94.8	78.4
Redundancy	3.5	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	295	0.8M sodium citrate, 0.8M ammonium sulfate, 0.5mM beta-heptyl thioglucoside, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K
1	VAPOR DIFFUSION, SITTING DROP	5	295	0.8M sodium citrate, 0.8M ammonium sulfate, 0.5mM beta-heptyl thioglucoside, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K