2DPS

Structure of Leucyl/phenylalanyl-tRNA-protein transferase

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2005-07-29
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97914, 0.97944, 0.98260
Spacegroup name	P 21 21 2
Unit cell lengths	114.042, 129.627, 38.751
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.850 - 2.400
R-factor	0.22
Rwork	0.220
R-free	0.27200
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.360	2.360
Rmerge	0.066	0.296
Number of reflections	24237
Completeness [%]	98.8	92.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.1	297.3	50mM HEPES, 0.2M tri-sodium citrate, 17%(v/v) polyethyleneglycol 3550, pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 297.3K
1	VAPOR DIFFUSION, HANGING DROP	8.1	297.3	50mM HEPES, 0.2M tri-sodium citrate, 17%(v/v) polyethyleneglycol 3550, pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 297.3K