2DPF
Crystal Structure of curculin1 homodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL24XU |
| Synchrotron site | SPring-8 |
| Beamline | BL24XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-12-08 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.827 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.996, 54.249, 84.055 |
| Unit cell angles | 90.00, 104.12, 90.00 |
Refinement procedure
| Resolution | 38.850 - 1.500 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kj1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.500 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | d*TREK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.050 | 0.470 |
| Number of reflections | 87424 | |
| <I/σ(I)> | 8.3 | 1.4 |
| Completeness [%] | 97.8 | 92 |
| Redundancy | 2.55 | 2.21 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3 | 298 | 5% PEG 8000, 0.25M lithium sulfate, 0.1M citrate , pH 3.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
