2DOQ
crystal structure of Sfi1p/Cdc31p complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-21 |
| Detector | ADSC |
| Wavelength(s) | 0.9794, 0.9393 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 87.283, 92.961, 189.358 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 94.490 - 3.000 |
| R-factor | 0.26057 |
| Rwork | 0.259 |
| R-free | 0.29899 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.253 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SnB |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.490 | 3.430 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Rmerge | 0.110 | 0.480 |
| Number of reflections | 12505 | |
| <I/σ(I)> | 3.8 | 1.3 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 6.8 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 277 | 9% iso-propanol, 0.1M MES pH=6.2, 0.2M Ca(OAc)2, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
