2DOK
Crystal structure of the PIN domain of human EST1A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2003-11-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.326, 51.586, 100.480 |
| Unit cell angles | 90.00, 119.25, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.21503 |
| Rwork | 0.213 |
| R-free | 0.25384 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.497 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 45150 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 278 | 1.26M (NH4)2SO4, 0.1M CHES-NaOH, 0.2M NaCl, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 278.0K |
