2DKJ
Crystal Structure of T.th.HB8 Serine Hydroxymethyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-09 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.75 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.988, 82.884, 94.074 |
| Unit cell angles | 90.00, 91.67, 90.00 |
Refinement procedure
| Resolution | 42.000 - 1.150 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| R-free | 0.19000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kkj |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.500 | 1.190 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Number of reflections | 302432 | |
| Completeness [%] | 95.8 | 90.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.8M lithium sulfate, 0.4M ammonium sulfate, 0.1M sodium citrate , pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
