2DKB

DIALKYLGLYCINE DECARBOXYLASE STRUCTURE: BIFUNCTIONAL ACTIVE SITE AND ALKALI METAL BINDING SITES

Experimental procedure

Spacegroup name	P 64 2 2
Unit cell lengths	152.700, 152.700, 86.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.100
R-factor	0.178
RMSD bond length	0.011
RMSD bond angle	2.200
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	2.100 *
Rmerge	0.078 *
Number of reflections	32665 *
Completeness [%]	93.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *		taken from Toney, M.D., (1991) J.Mol.Biol., 222, 873. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20-60 (mg/ml)
10	1	reservoir	sodium azide	0.02 (%)
2	1	drop	potassium phosphate	5 (mM)
3	1	drop	PLP	0.015 (mM)
4	1	drop	sodium azide	0.02 (%(w/v))
5	1	reservoir	morpholinoethanesulfonic acid	15 (mM)
6	1	reservoir	PEG4000	15 (%(w/v))
7	1	reservoir	potassium hydroxide		to neutralize
8	1	reservoir	sodium pyruvate	150 (mM)
9	1	reservoir	PLP	0.015 (mM)