2DIE
Alkaline alpha-amylase AmyK from Bacillus sp. KSM-1378
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-12-01 |
| Detector | MAC Science DIP-2030 |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 174.577, 41.467, 74.855 |
| Unit cell angles | 90.00, 92.24, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.100 |
| R-factor | 0.182 |
| Rwork | 0.177 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bli |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.230 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 28450 | |
| Completeness [%] | 91.3 | 90.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.05M sodium acetate buffer, 0.05M imidazole, 1%(w/v) protein, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
