2DI3

Crystal structure of the transcriptional factor CGL2915 from Corynebacterium glutamicum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-09
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000
Spacegroup name	C 2 2 21
Unit cell lengths	95.750, 107.634, 104.838
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.050
Rwork	0.205
R-free	0.25000
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.110
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.890	2.120
High resolution limit [Å]	2.050	2.050
Rmerge	0.074	0.383
Number of reflections	33895
<I/σ(I)>	19.6	5
Completeness [%]	98.7	99.7
Redundancy	5.4	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.8	296	30% PEG 4000, 0.1M NaOAc, 0.1M Tris-HCl, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 296K
1	VAPOR DIFFUSION, HANGING DROP	8.8	296	30% PEG 4000, 0.1M NaOAc, 0.1M Tris-HCl, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 296K