2DH6
Crystal structure of E. coli Apo-TrpB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-26 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 110.886, 110.886, 102.213 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.820 - 3.000 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CHAIN B of 1BKS |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.700 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.410 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.074 | 0.389 |
| Number of reflections | 13266 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.2 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | lithium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
