2DH2
Crystal Structure of human ED-4F2hc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.674, 67.846, 73.949 |
| Unit cell angles | 90.00, 98.49, 90.00 |
Refinement procedure
| Resolution | 17.830 - 2.100 |
| R-factor | 0.17777 |
| Rwork | 0.175 |
| R-free | 0.22969 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uok 1JI2 with Caspr MODELLER NORMAL MODES |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.446 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CaspR |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.800 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 22652 | |
| <I/σ(I)> | 14 | 2 |
| Completeness [%] | 99.5 | 98.6 |
| Redundancy | 4.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 25% PEG 4000, 0,2M SODIUM ACETATE, 0,1M TRIS BUFFER, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
