2DGL
Crystal structure of Escherichia coli GadB in complex with bromide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2004-08-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 297.051, 297.051, 233.731 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 3.150 |
| Rwork | 0.218 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pmm |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.270 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.260 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Number of reflections | 104068 | |
| <I/σ(I)> | 9.4 | 2.7 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 20% PEG 2000 MME, 0.18M potassium bromide, 0.1M sodium acetate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
