2DGJ
Crystal structure of EbhA (756-1003 domain) from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-30 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.97904 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 45.265, 161.848, 133.366 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.800 - 2.350 |
| R-factor | 0.241 |
| Rwork | 0.241 |
| R-free | 0.28700 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 20244 | |
| Completeness [%] | 96.4 | 89.4 |
| Redundancy | 6.1 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 0.1M acetate, 2.3M ammonium sulfate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
