2DGE
Crystal structure of oxidized cytochrome C6A from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.443, 51.843, 53.685 |
| Unit cell angles | 82.51, 62.08, 63.26 |
Refinement procedure
| Resolution | 31.470 - 1.500 |
| R-factor | 0.18767 |
| Rwork | 0.186 |
| R-free | 0.21451 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gdv |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.122 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.040 | 0.183 |
| Number of reflections | 59952 | |
| <I/σ(I)> | 20.9 | |
| Completeness [%] | 88.3 | 66.9 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEGMME550, MES, ZnSO4, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
