2DG0
Crystal structure of Drp35, a 35kDa drug responsive protein from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-26 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97908 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.089, 146.062, 151.947 |
| Unit cell angles | 90.00, 93.39, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.400 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.22200 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 145982 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.3 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 293 | 0.1M Tris, 23% PEG4000, 0.1M lithium sulfate, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
