2DFV
Hyperthermophilic threonine dehydrogenase from Pyrococcus horikoshii
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9796 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 144.433, 144.433, 305.069 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 2.050 |
| R-factor | 0.20568 |
| Rwork | 0.204 |
| R-free | 0.23878 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.708 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 1572057 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 40% PEG 400, 0.2M sodium chloride, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
