2DFB
Xylanase II from Tricoderma reesei at 100K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-18B |
Synchrotron site | Photon Factory |
Beamline | BL-18B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-11-22 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.15 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.970, 58.510, 69.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.800 - 1.110 |
R-factor | 0.108 |
Rwork | 0.108 |
R-free | 0.12100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1enx |
RMSD bond length | 0.012 |
RMSD bond angle | 0.028 |
Data reduction software | TRUNCATE |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.800 | 1.170 |
High resolution limit [Å] | 1.110 | 1.110 |
Rmerge | 0.066 | 0.203 |
Number of reflections | 77541 | |
Completeness [%] | 96.9 | 86.5 |
Redundancy | 6.6 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 298 | 0.4M ammonium sulfate, 0.1M bicine, 0.15M sodium iodide, 1.6% protein, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |