2DEY
Crystal structure of human peptidylarginine deiminase 4 in complex with histone H4 N-terminal tail including Arg3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 273 |
Detector technology | CCD |
Collection date | 2004-04-14 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 146.192, 60.611, 115.203 |
Unit cell angles | 90.00, 124.18, 90.00 |
Refinement procedure
Resolution | 33.570 - 2.250 |
R-factor | 0.20443 |
Rwork | 0.199 |
R-free | 0.24846 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wda |
RMSD bond length | 0.014 |
RMSD bond angle | 1.622 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.570 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 39243 | |
Completeness [%] | 98.3 | 98.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1M Imidazole (pH8.0), 0.2M lithium sulfate, 10% PEGMME2000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |