2DEW
Crystal structure of human peptidylarginine deiminase 4 in complex with histone H3 N-terminal tail including Arg8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 146.268, 60.773, 115.146 |
| Unit cell angles | 90.00, 124.26, 90.00 |
Refinement procedure
| Resolution | 38.340 - 2.100 |
| R-factor | 0.20575 |
| Rwork | 0.202 |
| R-free | 0.24078 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wda |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.465 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.340 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 48003 | |
| Completeness [%] | 97.9 | 97.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1M Imidazole (pH8.0), 0.2M lithium sulfate, 10% PEGMME2000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
