2DEO
1510-N membrane protease specific for a stomatin homolog from Pyrococcus horikoshii
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-19 |
| Detector | ADSC QUAMTUM 4r |
| Wavelength(s) | 0.9789, 0.9792, 0.9900 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.212, 108.400, 59.342 |
| Unit cell angles | 90.00, 110.06, 90.00 |
Refinement procedure
| Resolution | 19.760 - 3.000 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.24200 |
| Structure solution method | MAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.067 | 0.432 |
| Number of reflections | 12407 | |
| <I/σ(I)> | 25.2 | 3.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 21% PEG8000, 0.1M ammonium sulfate, 0.1M sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
