2DEI
Crystal Structure of galaktokinase from Pyrococcus horikoshii with AMP-PNP and galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-06-26 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.333, 39.067, 81.418 |
| Unit cell angles | 90.00, 109.50, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.700 |
| R-factor | 0.17897 |
| Rwork | 0.177 |
| R-free | 0.22132 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2cz9 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.395 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.086 | 0.364 |
| Number of reflections | 34247 | |
| <I/σ(I)> | 12.7 | 3.2 |
| Completeness [%] | 98.9 | 99.3 |
| Redundancy | 3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 295 | 2.5% PEG 4000, 50mM magnesium chloride, 0.1M MES, pH 6.3, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
