2DEH
Crystal structure of tt0972 protein form Thermus Thermophilus with Cl(-) ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-28 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.48521 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 65.171, 65.171, 202.364 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.980 - 2.450 |
| R-factor | 0.243 |
| Rwork | 0.243 |
| R-free | 0.27900 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | Mn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.053 | 0.498 |
| Number of reflections | 16915 | |
| <I/σ(I)> | 47.6 | 5.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 14.8 | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 6% PEG 4000, 60mM sodium acetate, 60mM lithium sulfate, 0.5mM nickel chloride, 30mM TRIS, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
