2DE7

The substrate-bound complex between oxygenase and ferredoxin in carbazole 1,9a-dioxygenase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-27
Detector	ADSC QUANTUM 4
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	97.862, 89.403, 104.665
Unit cell angles	90.00, 104.18, 90.00

Refinement procedure

Resolution	41.910 - 2.000
R-factor	0.207
Rwork	0.207
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vck 1ww9
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.910	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	118094
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.1M ammonium acetate, 12.5% PEG 3350, 0.05M Bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K