2DDQ
Crystal Structure of the Fab fragment of a R310 antibody complexed with (R)-HNE-histidine adduct
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Detector technology | CCD |
| Collection date | 2004-10-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.318, 65.794, 64.310 |
| Unit cell angles | 90.00, 118.77, 90.00 |
Refinement procedure
| Resolution | 35.830 - 2.350 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.900 | |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 18734 | |
| Completeness [%] | 95.8 | 95.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
