2DDO
Crystal Structure of Pyridoxal Kinase from the Escherichia coli pdxK gene at 2.6 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-04-10 |
| Detector | RIGAKU RAXIS II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.128, 74.356, 107.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.940 - 2.600 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.23800 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2ddm |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | bioteX |
| Data scaling software | bioteX |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.200 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.070 | 0.222 |
| Number of reflections | 16514 | |
| <I/σ(I)> | 9.9 | 2.3 |
| Completeness [%] | 99.2 | 98.2 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 21% PEG 4000, 100mM Tris-HCl, 200mM Na acetate, 40mM MgSO4, 10% glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
