2DD9

A mutant of GFP-like protein from Chiridius poppei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-03-07
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	108.750, 113.990, 133.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.810 - 2.300
R-factor	0.185
Rwork	0.185
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.500
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CNX
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.420
High resolution limit [Å]	2.300	2.300
Number of reflections	74303
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10.5	291	0.1M CAPS (pH 10.5), 2.6M Ammonium sulfate, 0.2M Sodium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 291K