2DCJ
A two-domain structure of alkaliphilic XynJ from Bacillus sp. 41M-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-16 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 122.927, 122.927, 331.943 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.390 - 2.240 |
| R-factor | 0.16048 |
| Rwork | 0.159 |
| R-free | 0.19116 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qh7 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.013 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.390 | 2.320 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Number of reflections | 72194 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 296 | 0.1M Hepes buffer pH 8.0, 0.35M Sodium citrate, 10% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
