2DC4
Structure of PH1012 protein from Pyrococcus Horikoshii OT3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 |
| Unit cell lengths | 53.123, 53.123, 111.147 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.560 - 1.650 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yem |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.010 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.085 | 0.402 |
| Number of reflections | 40754 | |
| <I/σ(I)> | 23.1 | 2.63 |
| Completeness [%] | 96.5 | 78.5 |
| Redundancy | 4.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.62 | 295 | 27.5w/w(%) PEG4000, 0.1M Mes, NaOH, pH 6.62, microbatch, temperature 295.0K |
