2DC3
Crystal structure of human cytoglobin at 1.68 angstroms resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45PX |
| Synchrotron site | SPring-8 |
| Beamline | BL45PX |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2004-07-02 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.530, 52.760, 83.440 |
| Unit cell angles | 90.00, 87.75, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.680 |
| R-factor | 0.142 |
| Rwork | 0.140 |
| R-free | 0.18100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v5h |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.558 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.740 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Number of reflections | 39043 | |
| <I/σ(I)> | 20.92 | 4.03 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 4.9 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1.4M sodium acetate, 100mM sodium cacodylate, 5mM KCN, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
