2DBN
Crystal Structure of a Hypothetical Protein JW0805 at High pH from Escherichia coli
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-20 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.97903, 0.97940, 0.964 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 69.688, 69.688, 174.649 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.900 - 1.700 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.20000 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 55165 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10.5 | 293 | 1.2M NaH2PO4, 0.8M K2HPO4, 0.1M CAPS buffer pH 10.5, 0.2M Li2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
