2DB7
Crystal structure of hypothetical protein MS0332
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-18 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.9789, 0.9791, 0.9650 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 28.322, 44.810, 92.088 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.070 - 1.900 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.22000 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 9565 | |
| <I/σ(I)> | 19.1 | 10.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.4 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.2 | 293 | PEG3350, Magnesium Chloride, pH 9.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
