2D7V
Structure of OsmC-like Protein of Unknown Function VCA0330 from Vibrio cholerae O1 biovar eltor str. N16961
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 150 |
Detector technology | CCD |
Collection date | 2005-02-15 |
Detector | SBC-2 |
Wavelength(s) | 0.97945 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 148.507, 40.023, 56.231 |
Unit cell angles | 90.00, 108.89, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.970 |
R-factor | 0.20218 |
Rwork | 0.199 |
R-free | 0.25846 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.626 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 1.970 | 1.970 |
Number of reflections | 23797 | |
Completeness [%] | 76.2 | 86.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 298 | 1.0M (NH4)2HPO4, 0.1M Acetate pH 4.5, pH 7.5, VAPOR DIFFUSION, temperature 298K |