2D58
Human microglia-specific protein Iba1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 60.748, 36.617, 44.024 |
| Unit cell angles | 90.00, 99.71, 90.00 |
Refinement procedure
| Resolution | 31.240 - 1.900 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.240 | 2.020 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.760 | |
| Number of reflections | 7462 | |
| Completeness [%] | 97.4 | 90.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.3 | 300 | 20% (w/v) polyethylene glycol (PEG) 4000, 10% (w/v) 2-propanol, 0.01M NiCl2 in 100mM of Tris-HCl (pH 7.3), VAPOR DIFFUSION, temperature 300K |
