2D55
Structural, physical and biological characteristics of RNA.DNA binding agent N8-actinomycin D
Replaces: 1D55Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Detector technology | DIFFRACTOMETER |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 51.370, 62.090, 25.040 |
| Unit cell angles | 90.00, 113.80, 90.00 |
Refinement procedure
| Resolution | 7.000 - 3.000 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 4.800 |
| Refinement software | X-PLOR |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 4 * | Kamitori, S., (1992) J.Mol.Biol., 225, 445. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | NA CACODYLATE | ||
| 5 | 1 | 1 | SPERMINE_HCL | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | oligonucleotide | 2.0 (mM) | |
| 2 | 1 | drop | 10 (mM) | ||
| 3 | 1 | drop | cacodylate | 20 (mM) | |
| 4 | 1 | drop | spermine tetrachloride | 2 (mM) | |
| 5 | 1 | drop | AMD | 1.5 (mM) | |
| 6 | 1 | drop | MPD | 7.5 (%(v/v)) | |
| 7 | 1 | reservoir | 15 (%) |
